Advanced Research Assistant - Maternity Leave Cover
The Parasites and Microbes Programme (PaM) seeks to appoint an Advanced Research Assistant to work within their laboratories in Nick Thomson’s faculty group (T216) at the Wellcome Sanger Institute. We use genomic approaches to explore questions of basic science relating to the evolution and spread of bacterial pathogens, and to understand how genotypes and phenotypes relate to each other in both pathogens and hosts.
About the Role:
This is a laboratory-based role, in which you will be responsible for performing both routine techniques and bespoke experimental processes. We seek an individual experienced in culturing bacterial and eukaryotic cells, who wants to contribute to developing cutting-edge infection models and culture systems. You will have opportunities to differentiate iPSC derived small intestinal organoids, and use them to generate and validate data obtained from phenotypic experiments.
You will work as part of our team to grow, maintain, and support strategically important tissue culture cell lines, infection models, and bacterial strains. You will perform infection experiments using bacterial pathogens and toxins, to explore host and pathogen response to infection. You will maintain and conduct experiments with organoids, including those derived from induced pluripotent stem cells (iPSCs). You will conduct a wide range of technically challenging procedures with these cells and organisms, and there will be exciting opportunities to contribute to the research relying on these tissue culture systems. A wide range of complex experimental methodologies will be used to drive cutting-edge research into the biology of human hosts and bacterial pathogens.
- Bachelor’s degree in Biological Sciences or relevant laboratory/research experience
- Experience working with mammalian cell lines in vitro
- Experience with core molecular biology techniques, such as RNA/DNA extraction, protein expression and purification, RT-PCR, qPCR, library preparation reactions
- Experience in planning and running experiments over several days
- Ability to develop, optimise, deploy, and document laboratory protocols
Competencies and Behaviours:
- Ability to work effectively, both independently and within a team, contributing to discussions and disseminating knowledge and information
- Meticulous attention to detail
- Excellent organisational and communication skills
Please include a CV and cover letter with your application. Your cover letter should outline your suitability for the role, referring specifically to the criteria in this advertisement and in the attached job description.
Applications will be considered on an ongoing basis and the role may close early if a successful appointment is made.
We are ideally looking for you to start Mid-Late September 2022.
We will consider this as a secondment opportunity, please seek your current line manager's approval before submitting an application.