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Advanced Research Assistant - CRISPR Human iPSC Gene Editing

Salary range £26,364 to £31,900 per annum plus benefits

We are seeking an enthusiastic individual to join our established team as an Advanced Research Assistant on a fixed term contract up to 12 months.

You'll perform bespoke genome engineering projects and develop gene modification techniques in human induced pluripotent stem cells (iPSCs) using CRISPR/Cas9 technology.

You will work as part of the human gene editing team within the Cellular Operations division, in the bespoke section led by Dr Andrew Bassett and in collaboration with other members of the scientific faculty at the Sanger Institute.

You will employ genome engineering techniques to model human diseases in iPS cells and their derivatives, and introduce or revert potential genetic changes to understand the causative lesions underlying such diseases.

The recent development of human iPSCs, and the use of genome editing technologies such as CRISPR/Cas9 has enabled us to model the genetic basis of many human diseases in an appropriate cell type derived from an individual with the disease. The Wellcome Sanger Institute has an extraordinary background in human disease genetics and has recently produced hundreds of iPSC cell lines derived from human patients with and without disease. 

We consider applications equally from those who wish to work full time (37 hours per week) or for a minimum of 4 days (30 hours per week) via a flexible working arrangement

Job Reference

Essential Skills

  • Science degree in biological sciences or extensive, practical experience
  • Minimum of 3 years of working laboratory experience
  • Practical experience of mammalian cell culture
  • Theoretical understanding of genome engineering techniques including CRISPR/Cas9
  • Understanding of genotyping techniques (e.g. qPCR, HRMA)
  • Experience in interpreting gene structures on standard genome browsers (e.g. UCSC, Ensembl)
  • Ability to test and implement new approaches and techniques including troubleshooting
  • Capable of working to a schedule and delivery of a variety of challenging projects at a time within agreed deadlines
  • Ability to work both as part of a team and also independently once appropriate training and mentorship have been provided
  • Good communication skills to allow accurate information to be exchanged
  • Good computer skills in the use of office software (e.g. Excel, Word, Powerpoint)

Ideal Skills

  • Experience of human ES or iPS cell culture
  • An excellent understanding and practical experience in molecular biology strategies and techniques, including molecular cloning, nucleic acid isolation and purification, and PCR.
  • Practical experience of genome engineering techniques including CRISPR/Cas9
  • Practical experience of genotyping techniques (e.g. qPCR, HRMA)
  • Use of sequence manipulation software (e.g. Vector NTI, SnapGene, etc.)

Other information

The Wellcome Sanger Institute is a charitably funded research centre and committed to training the next generation of genome scientists. Passionate about understanding the role of genetics in health and disease and a world leader in the genomic revolution, our mission is to use genome sequences to advance understanding of human and pathogen biology in order to improve human health.

Our Campus: Set over 125 acres, the stunning and dynamic Wellcome Genome Campus is the biggest aggregate concentration of people in the world working on the common theme of Genomes and BioData. It brings together a diverse and exceptional scientific community, committed to delivering life-changing science with the reach, scale and imagination to pursue some of humanity’s greatest challenges.

Our Benefits: Our employees have access to a comprehensive range of benefits and facilities including:

  • Group Defined Contribution Pension Scheme and Life Assurance
  • Group Income Protection
  • Private Health Insurance
  • 25 days annual leave, increasing by one day a year to a maximum of 30
  • Family friendly environment including options for flexible and part-time working, a childcare voucher scheme and Campus Nursery
  • Two days paid Employee Volunteering Leave a year
  • Employee Discount Scheme
  • Campus Gym, tennis courts, cricket pitch and sports hall plus a range of dining facilities
  • Active Campus Sports and Social Club
  • Free Campus Bus Service

Genome Research Limited is an Equal Opportunity employer. As part of our dedication to gender equality and promoting women’s careers in science, we hold an Athena SWAN Bronze Award. We will consider applicants without discrimination on grounds of disability, sexual orientation, pregnancy or maternity leave status, race or national or ethnic origin, age, religion or belief, gender identity or re-assignment, marital or civil partnership status, protected veteran status (if applicable) or any other characteristic protected by law.

Please include a covering letter and CV with your application

Closing date for applications: 31st March 2018