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Advanced Research Assistant: CRISPR editing PSCs to understand adipocyte biology

Salary in the region of £26,693 - £32,299 (dependent on experience) plus excellent benefits

Fixed term for 3 years

We are seeking an enthusiastic, motivated advanced research assistant to generate genetically engineered human pluripotent stem cells (PSCs) (both knockout and point mutations) using CRISPR/Cas9 technology to identify and model the causal genetic variants underlying metabolic diseases such as obesity and associated complications.

You will target genes with a potential role in white and brown human adipocyte differentiation and function identified from human and mouse genetic studies. They will employ differentiation protocols developed in our lab to understand the role of these variants during adipocytes differentiation and mature adipocytes function.

You will also have the opportunity to apply and develop cutting edge genome engineering techniques, including the use and analysis of whole genome CRISPR knockout libraries for forward genetic screens for the identification of new candidate genes involved in human adipocyte biology.

The job will involve collaborative work with the human gene editing research and development team led by Dr Andrew Bassett who are performing such modifications routinely, and allow the scope to develop and improve methods for genetic engineering and screening of these cells.

Job Reference

Essential Skills

  • Science degree in biological sciences or equivalent or extensive, proven practical experience 
  • Minimum of 3 years of working laboratory experience 
  • Practical experience in mammalian cell culture 
  • Practical experience and theoretical understanding of genome engineering techniques including CRISPR/Cas9
  • Theoretical understanding of genotyping techniques (e.g. NGS amplicon sequencing) and/or stem cell differentiation
  • Experience in interpreting gene structures on standard genome browsers 
  • Ability to test and implement new approaches and techniques including troubleshooting 
  • Capable of working to a schedule and delivery of a variety of challenging projects simultaneously within agreed deadlines
  • Ability to work both as part of a team and also independently once appropriate training and guidance have been provided
  • Excellent laboratory practice 
  • Excellent work organisation practice 
  • Good communication skills to allow accurate information to be exchanged 
  • Good computer skills in the use of office software (e.g. Excel, Word, Powerpoint) 

Ideal Skills

  • Practical experience in human ES or iPS cells culture
  • An excellent understanding and practical experience in molecular biology strategies and techniques, including molecular cloning, nucleic acid isolation and purification, and PCR. 
  • Experience in the use of sequence manipulation software (e.g. Vector NTI, SnapGene, etc.) 
  • Practical experience of genotyping techniques (e.g. NGS amplicon sequencing) and/or stem cell differentiation

Other information

The Wellcome Sanger Institute is a charitably funded research centre and committed to training the next generation of genome scientists. Focused on understanding the role of genetics in health and disease and a world leader in the genomic revolution, our mission is to use genome sequences to advance understanding of human and pathogen biology in order to improve human health. We aim to provide results that can be translated into diagnostics, treatments or therapies that reduce global health burdens. Our science is large-scale and organised into Programmes, led by our Faculty who conceive and deliver our science, and supported by our Scientific Operations teams responsible for all data production pipelines at the Institute.

Our Campus: Set over 125 acres, the stunning and dynamic Wellcome Genome Campus is the biggest aggregate concentration of people in the world working on the common theme of Genomes and BioData. It brings together a diverse and exceptional scientific community, committed to delivering life-changing science with the reach, scale and imagination to pursue some of humanity’s greatest challenges.

Our Benefits: Our employees have access to a comprehensive range of benefits and facilities including:

  • Group Defined Contribution Pension Scheme and Life Assurance
  • Group Income Protection
  • Private Health Insurance
  • 25 days annual leave, increasing by one day a year to a maximum of 30
  • Family friendly environment including options for flexible and part-time working, a childcare voucher scheme, Campus Nursery and Summer holiday club
  • Two days paid Employee Volunteering Leave a year
  • Employee Discount Scheme
  • Campus Gym, tennis courts, cricket pitch and sports hall plus a range of dining facilities
  • Active Campus Sports and Social Club
  • Free Campus Bus Service

Genome Research Limited is an Equal Opportunity employer. As part of our commitment to equality, diversity and inclusion and promoting equality in careers in science, we hold an Athena SWAN Bronze Award and have an active Equality, Diversity and Inclusion programme of activity. We will consider all applicants without discrimination on grounds of disability, sexual orientation, pregnancy or maternity leave status, race or national or ethnic origin, age, religion or belief, gender identity or re-assignment, marital or civil partnership status, protected veteran status (if applicable) or any other characteristic protected by law. We are open to a range of UK-based flexible working options including part-time or full-time employment as well as flexible hours due to caring or other commitments.

Please include a covering letter and CV with your application.  Closing date: 30th June 2018however applications will be considered and reviewed on an on-going basis and therefore the post may be filled before the deadline.