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Advanced Research Assistant - Mouse Pipelines Genome Engineering

Salary in the region of £26,693 to £32,299 per annum (depending on experience), plus benefits 

The Sanger Mouse Pipelines Genome Engineering group (GE) generates custom mutations in mice by gene targeting in embryonic stem (ES) cells and directly in the mouse zygote by applying cutting edge gene editing technology. GE creates mutant alleles of interest to Sanger Institute Faculty, many of which are used as models for human disease. Our efforts benefit from extensive targeting vector/ES cell modular resources generated previously as part of the KOMP, EUCOMM, and EUCOMMTOOLs high throughput gene targeting programs and associated expertise as well as the successful implementation of CRISPR/Cas9 methods.

We are seeking a highly motivated and flexible individual to join the GE team as an Advanced Research Assistant. Your main responsibilities will be execution of day-to-day workflows of projects for the generation of targeting vectors, CRISPR/Cas9 reagents and the generation and validation of quality assured targeted mouse ES cells. Key responsibilities include execution and timely delivery of these resources, accurate record keeping, project troubleshooting and an ability to work across multiple projects.  In addition, the group also has a research and development (R&D) commitment to implement new approaches that facilitate the insertion of complex alleles directly into the mouse zygote.

Previous experience of mammalian cell culture, especially mouse ES cell (mESC) culture is a prerequisite for this role. You would also be expected to be able to genotype the mESC clones generated in order to provide quality assured reagents for microinjection. The candidate should have a broad knowledge of molecular biology techniques such as nucleic acid extraction, construct generation (via conventional cloning, Gibson assembly and/or recombineering), PCR, restriction enzyme analysis and sequencing. An understanding of conventional gene targeting and the more recent CRISPR/Cas9 genome engineering techniques would be advantageous.

You must be able to work as part of a team and also have the ability to independently handle complex workflows from project start to delivery. Good communication/record keeping skills are a key part of the role.

Some weekend cell culture is anticipated in this role.

Job Reference
83148

Essential Skills

  • Science degree in biological sciences/molecular biology or extensive, proven practical experience in a relevant area.
  • Experienced in mammalian cell culture, especially routine mouse ES cells culturing.
  • An understanding and practical experience in molecular biology strategies and techniques, including some of the following: molecular cloning, construct generation (via conventional cloning, Gibson assembly and recombineering), PCR, restriction enzyme analysis and sequencing.
  • Isolation and purification of nucleic acid, particularly RNA based methodologies for the generation of CRISPR reagents.
  • Practical experience and theoretical understanding of genotyping techniques, including qPCR, long range PCR and sequencing and data interpretation there of.
  • Capable of performing/tracking a variety of challenging projects simultaneously
  • Ability to work as part of a team and also independently once appropriate training and guidance has been provided.
  • Good communication skills to allow accurate information to be exchanged
  • Ability to schedule and deliver simultaneous projects within agreed deadlines
  • Good computer skills

Ideal Skills

  • Experience of transfection, nucleofection and electroporation technologies would be advantageous
  • Practical experience in understanding gene structures on standard genome browsers
  • Practical experience and theoretical understanding of conventional and nuclease assisted gene targeting strategies
  • Ability to test ,implement and troubleshoot new approaches and techniques

Other information

Please include a covering letter and CV with your application.  

Closing date, 8th September 2018

Our Campus: Set over 125 acresthe stunning and dynamic Wellcome Genome Campus is the biggest aggregate concentration of people in the world working on the common theme of Genomes and BioData. It brings together a diverse and exceptional scientific community, committed to delivering life-changing science with the reach, scale and imagination to solve some of humanity’s greatest challenges.  

The Campus is a community of organisations working in genomic research, enterprise, education and discourse. It is home to some of the world's foremost institutes and organisations in genomics and computational biology, including the Wellcome Trust Sanger Institute which is the largest organisation on Campus, the European Bioinformatics Institute (EMBL-EBI), Open Targets and Genomics England.  Also located here is Connecting Science - its mission is to enable everyone to explore genomic science and its impact on research, health and society by drawing on the ground-breaking research taking place on the Campus.

Our Benefits: Our employees have access to a comprehensive range of benefits and facilities including:

  • Group Defined Contribution Pension Scheme and Life Assurance
  • Group Income Protection
  • Private Health Insurance
  • 25 days annual leave, increasing by one day a year to a maximum of 30
  • Family friendly environment including options for flexible and part-time working, a childcare voucher scheme and Campus Nursery
  • Two days paid Employee Volunteering Leave a year
  • Employee Discount Scheme
  • Campus Gym, tennis courts, cricket pitch and sports hall plus a range of dining facilities
  • Active Campus Sports and Social Club
  • Free Campus Bus Service

Genome Research Limited is an Equal Opportunity employer. As part of our dedication to gender equality and promoting women’s careers in science, we hold an Athena SWAN Bronze Award. We will consider all qualified individuals without discrimination on grounds of disability, sexual orientation, pregnancy or maternity leave status, race or national or ethnic origin, age, religion or belief, gender identity or re-assignment, marital or civil partnership status, protected veteran status (if applicable) or any other characteristic protected by law.